Translational Features of Human Alpha 2b Interferon Production in Escherichia coli-Reference-Cited by-同舟云学术

Translational Features of Human Alpha 2b Interferon Production in Escherichia coli

Published:2004-08 Issue:8 Volume:70 Page:5033-5036
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Valente C. A.¹,Prazeres D. M. F.¹,Cabral J. M. S.¹,Monteiro G. A.¹

Affiliation:

1. Centro de Engenharia Biológica e Química, Instituto Superior Técnico, 1049-001 Lisbon, Portugal

Abstract

ABSTRACT The yield of human alpha 2b interferon in Escherichia coli was optimized by replacement of low-usage arginine codons located in the mRNA 5′ end. The differences observed among the various gene variants suggest that codon usage, Shine-Dalgarno-like sequences, and mRNA secondary structure contribute to the performance of E. coli translation machinery.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.8.5033-5036.2004

Reference17 articles.

1. Alexandrova R., M. Eweida, F. Georges, B. Dragulev, M. AbouHaidair, and I. Ivanov. 1995. Domains in human interferon alpha-1 gene containing tandems of arginine codons AGG play the role of translational initiators in Escherichia coli. Int. J. Biochem. Cell Biol.27:469-473.

2. Bonekamp, F., and K. F. Jensen. 1988. The AGG codon is translated slowly in E. coli even at very low expression levels. Nucleic Acids Res.16:3013-3024.

3. Bordens, R., S. E. Grossberg, P. P. Trotta, and T. L. Nagabbushan. 1997. Molecular and biologic characterization of recombinant interferon-a2b. Semin. Oncol.24:S9-41-S9-51.

4. Brinkmann, U., R. E. Mattes, and P. Buckel. 1989. High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product. Gene85:109-114.

5. Chen, G. F., and M. Inouye. 1990. Suppression of the negative effect of minor arginine codons on gene expression; preferential usage of minor codons within the first 25 codons of the Escherichia coli genes. Nucleic Acids Res.18:1465-1473.

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