Affiliation:
1. Department of Biological Sciences
2. Department of Biochemistry, University of Iowa, Iowa City, Iowa
Abstract
ABSTRACT
Yeast Sln1p is an osmotic stress sensor with histidine kinase activity. Modulation of Sln1 kinase activity in response to changes in the osmotic environment regulates the activity of the osmotic response mitogen-activated protein kinase pathway and the activity of the Skn7p transcription factor, both important for adaptation to changing osmotic stress conditions. Many aspects of Sln1 function, such as how kinase activity is regulated to allow a rapid response to the continually changing osmotic environment, are not understood. To gain insight into Sln1p function, we conducted a two-hybrid screen to identify interactors. Mog1p, a protein that interacts with the yeast Ran1 homolog, Gsp1p, was identified in this screen. The interaction with Mog1p was characterized in vitro, and its importance was assessed in vivo.
mog1
mutants exhibit defects in SLN1-SKN7 signal transduction and mislocalization of the Skn7p transcription factor. The requirement for Mog1p in normal localization of Skn7p to the nucleus does not fully account for the
mog1
-related defects in SLN1-SKN7 signal transduction, raising the possibility that Mog1p may play a role in Skn7 binding and activation of osmotic response genes.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Reference65 articles.
1. Alberts, A. S., N. Bouquin, L. H. Johnston, and R. Treisman. 1998. Analysis of RhoA-binding proteins reveals an interaction domain conserved in heterotrimeric G protein beta subunits and the yeast response regulator protein Skn7. J. Biol. Chem.273:8616-8622.
2. Alex, L. A., C. Korch, C. P. Selitrennikoff, and M. I. Simon. 1998. COS1, a two-component histidine kinase that is involved in hyphal development in the opportunistic pathogen Candida albicans. Proc. Natl. Acad. Sci. USA95:7069-7073.
3. Altered Phosphotransfer in an Activated Mutant of the
Saccharomyces cerevisiae
Two-Component Osmosensor Sln1p
4. Ausubel F. M. R. Brent R. E. Kingston D. E. Moore J. G. Seidman J. A. Smith and K. Struhl. 1989. Current protocols in molecular biology vol. 1. John Wiley & Sons Inc. New York N.Y.
5. Baker, R. P., M. T. Harreman, J. F. Eccleston, A. H. Corbett, and M. Stewart. 2001. Interaction between Ran and Mog1 is required for efficient nuclear protein import. J. Biol. Chem.276:41255-41262.
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