Elimination of overgrowth in delayed-incubation membrane filter test for total coliforms by m-ST holding medium

Author:

Chen M,Hickey P J

Abstract

Two holding media were compared for their effects on total coliform recovery by the delayed-incubation membrane filter procedure. LES-MF holding medium contains tryptone, m-Endo broth, dipotassium hydrogen phosphate, sodium benzoate, sulfanilamide, para-aminobenzoic acid, and cycloheximide (pH 7.0). m-ST holding medium contains ethanol, sodium monophosphate, dipotassium hydrogen phosphate, sulfanilamide, and Tris (pH 8.6). In tests with natural water and wastewater samples from various sources, recovery with LES-MF and m-ST were similar after a 1-day holding period. With LES-MF, however, after a 2- or 3-day holding period, coliform bacteria frequently were partially or totally overgrown by noncoliforms, causing significant reductions in coliform counts. No significant overgrowth was observed with m-ST. We propose that m-ST be used for all holding periods longer than 1 day.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference8 articles.

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4. Fleiss J. L. 1973. Statistical methods for rates and proportions. John Wiley & Sons Inc. New York.

5. Holding effects on coliform enumeration in drinking water samples;McDaniels A. E.;Appl. Environ. Microbiol.,1985

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