Growth of Fast- and Slow-Growing Rhizobia on Ethanol

Author:

Sadowsky Michael J.1,Bohlool B. Ben1

Affiliation:

1. Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822

Abstract

Free-living soybean rhizobia and Bradyrhizobium spp. (lupine) have the ability to catabolize ethanol. Of the 30 strains of rhizobia examined, only the fast- and slow-growing soybean rhizobia and the slow-growing Bradyrhizobium sp. (lupine) were capable of using ethanol as a sole source of carbon and energy for growth. Two strains from each of the other Rhizobium species examined ( R. meliloti, R. loti , and R. leguminosarum biovars phaseoli, trifolii , and viceae ) failed to grow on ethanol. One Rhizobium fredii (fast-growing) strain, USDA 191, and one (slow-growing) Bradyrhizobium japonicum strain, USDA 110, grew in ethanol up to concentrations of 3.0 and 1.0%, respectively. While three of the R. fredii strains examined (USDA 192, USDA 194, and USDA 205) utilized 0.2% acetate, only USDA 192 utilized 0.1% n -propanol. None of the three strains utilized 0.1% methanol, formate, or n -butanol as the sole carbon source.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference16 articles.

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2. Fred E. B. I. L. Baldwin and E. McCoy. 1932. Root nodule bacteria and leguminous plants. University of Wisconsin Studies in Science vol. 5. University of Wisconsin Press Madison.

3. The uptake and hydrolysis of disaccharides by fast- and slow-growing species of Rhizobium;Glenn A. R.;Arch. Microbiol.,1981

4. Oxidation of substrates by isolated bacteroids and free-living cells of Rhizobium leguminosarum 3841;Glenn A. R.;J. Gen. Microbiol.,1981

5. Diagnostic features in the characterization of the root-nodule bacteria of legumes;Graham P. H.;Plant Soil,1964

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