Author:
Tinglu G,Ghosh A,Ghosh B K
Abstract
Subcellular distribution of the alkaline phosphatase of Bacillus licheniformis 749/C was determined by an immunoelectron microscopy method. Anti-alkaline phosphatase antibody labeled with 15- to 18-nm colloidal gold particles (gold-immunoglobulin G [IgG] complex) were used for the study. Both the plasma membrane and cytoplasmic material were labeled with the gold-IgG particles. These particles formed clusters in association with the plasma membrane; in contrast, in the cytoplasm the particles were largely dispersed, and only a few clusters were found. The gold-IgG binding was quantitatively estimated by stereological analysis of labeled, frozen thin sections. This estimation of a variety of control samples showed that the labeling was specific for the alkaline phosphatase. Cluster formation of the gold-IgG particles in association with the plasma membrane suggests that existence of specific alkaline phosphatase binding sites (receptors) in the plasma membrane of B. licheniformis 749/C.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference27 articles.
1. Structural and functional evidence of cooperativity between membranes and cell wall in bacteria;Bayer M. E.;Int. Rev. Cytol.,1981
2. Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique;Bendayar M.;J. Histochem. Cytochem.,1983
3. Frozen thin sections of fresh tissue for electron microscopy, with a description of pancreas and liver;Christensen A. K.;J. Cell Biol.,1971
4. The application of analytical electron microscopy in the localization of individual LDL-binding sites on cell surfaces;de Bruijn W. C.;Artery,1980
5. Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions;Frens G.;Nature (London) Phys. Sci.,1973
Cited by
25 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献