Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of Acinetobacter baumannii

Author:

Gouby A1,Carles-Nurit M J1,Bouziges N1,Bourg G1,Mesnard R1,Bouvet P J1

Affiliation:

1. Faculte de Medecine, Institut National de la Santé et de la Recherche Médicale, Unité U 65, Montpellier-Nîmes, France.

Abstract

Genomic DNAs from taxonomically and epidemiologically well-defined strains of Acinetobacter baumannii were digested with restriction endonucleases that cleave with low frequency, and the fragments were separated by pulse-field gel electrophoresis. Restriction fragment length polymorphisms were observed. Restriction fragment length polymorphism analysis can be used as an epidemiological tool to delineate outbreaks of nosocomial infections caused by A. baumannii.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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