Affiliation:
1. Department of Microbial Ecology, University of Vienna, Althanstr. 14, 1090 Vienna, Austria
Abstract
ABSTRACT
Fluorescence
in situ
hybridization (FISH) with singly labeled rRNA-targeted oligonucleotide probes is widely applied for direct identification of microbes in the environment or in clinical specimens. Here we show that a replacement of singly labeled oligonucleotide probes with 5′-, 3′-doubly labeled probes at least doubles FISH signal intensity without causing specificity problems. Furthermore, Cy3-doubly labeled probes strongly increase
in situ
accessibility of rRNA target sites and thus provide more flexibility for probe design.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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