Two Photolyases Repair Distinct DNA Lesions and Reactivate UVB-Inactivated Conidia of an Insect Mycopathogen under Visible Light-Reference-Cited by-同舟云学术

Two Photolyases Repair Distinct DNA Lesions and Reactivate UVB-Inactivated Conidia of an Insect Mycopathogen under Visible Light

Published:2019-02-15 Issue:4 Volume:85 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Wang Ding-Yi¹,Fu Bo²,Tong Sen-Miao¹²,Ying Sheng-Hua¹,Feng Ming-Guang¹^ORCID

Affiliation:

1. Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China

2. College of Agricultural and Food Science, Zhejiang A&F University, Lin’an, Zhejiang, China

Abstract

Protecting fungal cells from damage from solar UVB irradiation is critical for development and application of fungal insecticides but is mechanistically not understood in Beauveria bassiana , a classic insect pathogen. We unveil that two intranuclear photolyases, Phr1 and Phr2, play essential roles in repairing UVB-induced dsDNA lesions through respective decomposition of cytotoxic cyclobutane pyrimidine dimers and (6-4)-pyrimidine-pyrimidine photoproducts, hence reactivating UVB-inactivated cells effectively under visible light. Our findings shed light on the high potential of both photolyase genes for use in improving UVB resistance and application strategy of fungal insecticides.

Funder

Fundamental Research Funds for the Central Universities of China

National Natural Science Foundation of China

Ministry of Science and Technology of the People's Republic of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02459-18

Reference48 articles.

1. Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types

2. Stress tolerance and virulence of insect-pathogenic fungi are determined by environmental conditions during conidial formation

3. Antioxidant enzymes and their contributions to biological control potential of fungal insect pathogens

4. Madronich S. 1993. UV radiation in the natural and perturbed atmosphere, p 17−69. In Tevini M (ed), UV-B radiation and ozone depletion. Lewis, Boca Raton, FL.

5. Effects of simulated solar radiation on conidial germination of Metarhizium anisopliae in different formulations

Cited by 36 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Rad6, a ubiquitin conjugator required for insect-pathogenic lifestyle, UV damage repair, and genomic expression of Beauveria bassiana;Microbiological Research;2024-04

2. Rad2, Rad14 and Rad26 recover Metarhizium robertsii from solar UV damage through photoreactivation in vivo;Microbiological Research;2024-03

3. High photoreactivation activities of Rad2 and Rad14 in recovering insecticidal Beauveria bassiana from solar UV damage;Journal of Photochemistry and Photobiology B: Biology;2024-02

4. Two sirtuin proteins, Hst3 and Hst4, modulate asexual development, stress tolerance, and virulence by affecting global gene expression in Beauveria bassiana;Microbiology Spectrum;2024-01-09

5. Inactivation of pathogenic microorganisms in water by electron beam excitation multi-wavelength ultraviolet irradiation: Efficiency, influence factors and mechanism;Journal of Environmental Management;2024-01