Affiliation:
1. Department of Biology, The Catholic University of America, Washington, D.C. 20017
Abstract
Auxotrophic mutants of
Hydrogenomonas eutropha
and
H. facilis
requiring utilizable amino acids were employed to demonstrate the simultaneous utilization of H
2
and an organic substrate for growth. The ratio of the cell yields under dual substrate conditions compared to heterotrophic conditions indicated the relative contributions of the autotrophic and heterotrophic systems to the growth of the organism. Wildtype
H. eutropha
grown under simultaneous conditions exhibited a dicyclic growth pattern, the first cycle representing either heterotrophic or simultaneous growth and the second cycle representing autotrophic growth. The duration of the changeover period was either very short with no plateau or long with a plateau up to 8 hr, depending upon the organic substrate. The growth rate under simultaneous conditions with some organic substrates was faster than either the autotrophic or heterotrophic rate, but was not the sum of the two rates. The data suggest that, in the presence of both organic and inorganic substrates, heterotrophic metabolism functions normally but autotrophic metabolism is partially repressed.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference16 articles.
1. The biochemistry of Hydrogenomonas. I. The hydrogenase of Hydrogenomonas facilis in cellfree preparations;ATKINSON D. E.;J. Biol. Chem.,1954
2. Physiologische Untersuchungen uber den chemolithotrophen Stoffweschsel neu isolierter Hydrogenomonas-Stzamme;BARTHA R.;Mikrobiol.,1962
3. Arch.
4. Phosphorylation in hydrogen bacteria;BONGERS L.;J. Bacteriol.,1967
5. Isolation of biochemically deficient mutants of bacteria by penicillin;DAVIS B. D.;J. Am. Chem. Soc.,1948
Cited by
23 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献