Affiliation:
1. Department of Molecular Biology, Graduate School of Science, Institute for Advanced Research, Nagoya University, and CREST, Japan Science and Technology Corporation, Chikusa-ku, Nagoya, Japan
Abstract
ABSTRACT
Cells of budding yeast give rise to mother and daughter cells, which differ in that only mother cells express the HO endonuclease gene and are thereby able to switch mating types. In this study, we identified the
MKT1
gene as a positive regulator of
HO
expression. The
MKT1
gene encodes a protein with two domains, XPG-N and XPG-I, which are conserved among a family of nucleases, including human XPG endonuclease. Loss of
MKT1
had little effect on
HO
mRNA levels but resulted in decreased protein levels. This decrease was dependent on the 3′ untranslated region of the
HO
transcript. We screened for proteins that associate with Mkt1 and isolated Pbp1, a protein that is known to associate with Pab1, a poly(A)-binding protein. Loss of
PBP1
resembles an
mkt1
Δ deletion, causing decreased expression of
HO
at the posttranscriptional level. Mkt1 and Pbp1 cosedimented with polysomes in sucrose gradients, with Mkt1 distribution in the polysomes dependent on Pbp1, but not vice versa. These observations suggest that a complex of Mkt1 and Pbp1 regulates the translation of
HO
mRNA.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
49 articles.
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