Simple screening method for beta-lactamase-positive and -negative ampicillin-resistant Haemophilus influenzae isolates

Author:

Parr T R,Pai C H,Bryan L E

Abstract

A simple, inexpensive method for screening for beta-lactamase-positive and beta-lactamase-negative ampicillin-resistant Haemophilus influenzae isolates was developed. Disks containing 10 micrograms of cloxacillin yielded no zone of inhibition when placed on chocolate agar plates inoculated with beta-lactamase-positive (16 strains) or ampicillin-resistant (greater than or equal to 1 microgram/ml) beta-lactamase-negative (10 strains) H. influenzae, whereas ampicillin-susceptible (less than or equal to 0.5 microgram/ml; 36 strains) H. influenzae almost always (92%) showed a zone of inhibition.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference16 articles.

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