Affiliation:
1. Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706
Abstract
Purified lipopolysaccharide vesicles dissociate when treated with ethylenediaminetetraacetic acid (EDTA) and then reassemble when dialyzed against Mg
2+
. Purified outer, lipopolysaccharide membrane (L membrane) is partially dissociated by treatment with EDTA and fully dissociated upon further treatment with Triton X-100. Both the partially and fully dissociated L membrane can be reassembled by dialysis against Mg
2+
. Reassembly of lipopolysaccharide or L membrane in the presence of intact flagella results in specific attachment of flagellar basal bodies to vesicles via the L and sometimes the M ring. Lipopolysaccharide and L membrane appear to be composed of substructures bound together by both Mg
2+
(divalent cation)-mediated and hydrophobic bonds.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
90 articles.
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