Multicenter Evaluation of Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) Spectroscopy-Based Method for Rapid Identification of Clinically Relevant Yeasts-Reference-Cited by-同舟云学术

Multicenter Evaluation of Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) Spectroscopy-Based Method for Rapid Identification of Clinically Relevant Yeasts

Published:2022-01-19 Issue:1 Volume:60 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Lam Lisa M. T.¹,Ismail Ashraf A.¹^ORCID,Lévesque Simon²³,Dufresne Simon F.⁴,Cheng Mathew P.⁵,Vallières Émilie⁶,Luong Me-Linh⁷,Sedman Jacqueline¹,Dufresne Philippe J.⁸

Affiliation:

1. McGill University, Sainte-Anne-de-Bellevue, Québec, Canada

2. CIUSSS de l’Estrie—CHUS, Sherbrooke, Québec, Canada

3. Université de Sherbrooke, Sherbrooke, Québec, Canada

4. CIUSSS de l’Est-de-l’île-de-Montréal—Hôpital Maisonneuve-Rosemont, Montréal, Québec, Canada

5. Centre Universitaire de Santé McGill, Montréal, Québec, Canada

6. Centre Hospitalier Universitaire Sainte-Justine, Montréal, Québec, Canada

7. Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada

8. Laboratoire de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Québec, Canada

Abstract

Fourier transform infrared (FTIR) spectroscopy has demonstrated applicability as a reagent-free whole-organism fingerprinting technique for both microbial identification and strain typing. For routine application of this technique in microbiology laboratories, acquisition of FTIR spectra in the attenuated total reflectance (ATR) mode simplifies the FTIR spectroscopy workflow, providing results within minutes after initial culture without prior sample preparation.

Funder

Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01398-21

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