Author:
Ferrante A,Rowan-Kelly B,Paton J C
Abstract
The effects of pneumolysin, a sulfhydryl-activated cytolytic toxin produced by Streptococcus pneumoniae, on the in vitro human lymphocyte response was examined. The toxin, at concentrations of one to five hemolytic units per ml, caused marked inhibition of the response of lymphocytes to concanavalin A, phytohemagglutinin, pokeweed mitogen, and protein A. The response was assessed by measuring both [3H]thymidine incorporation and the ability of lymphocytes to produce immunoglobulins and lymphokine activity. The effects of pneumolysin were irreversible, could be prevented by pretreatment of the toxin with cholesterol, and were not related to a direct cytotoxic effect on the lymphocytes. Pneumolysin appeared to act at the initiation phase of the immune response and had no effect on lymphocytes committed to DNA synthesis or to the synthesis and secretion of immunoglobulins. Furthermore, pneumolysin-mediated inhibition of the lymphocyte response was not due to the inhibition of binding of mitogens to leukocytes and is likely to be related to effects on membrane-mediated signals essential for lymphocyte triggering. This may be one means by which pneumolysin plays a role in the pathogenesis of pneumococcal infections.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference15 articles.
1. Some observations on the pneumococcus and on the current status of pneumococcal disease and its prevention;Austrian R.;Rev. Infect. Dis. 3:(Suppl.):1-17.,1981
2. Early decay of human neutrophil chemotactic responsiveness following isolation from peripheral blood;Ferrante A.;Clin. Exp. Immunol.,1979
3. Human neutrophils require activation by mononuclear leucocyte conditioned medium to kill the pathogenic free-living amoeba, Naegleriafowleri;Ferrante A.;Clin. Exp. Immunol.,1984
4. Suppression of immunological responses in mice by treatment with amphotericin B;Ferrante A.;Clin. Exp. Immunol.,1979
5. Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human blood by the Hypaque-Ficoll method;Ferrante A.;J. Immunol. Methods,1980
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