Direct Identification of Major Blood Culture Pathogens, Including Pseudomonas aeruginosa and Escherichia coli , by a Panel of Fluorescence In Situ Hybridization Assays Using Peptide Nucleic Acid Probes-Reference-Cited by-同舟云学术

Direct Identification of Major Blood Culture Pathogens, Including Pseudomonas aeruginosa and Escherichia coli , by a Panel of Fluorescence In Situ Hybridization Assays Using Peptide Nucleic Acid Probes

Published:2005-04 Issue:4 Volume:43 Page:1947-1949
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Søgaard M.¹,Stender H.²,Schønheyder H. C.¹

Affiliation:

1. Department of Clinical Microbiology, Aalborg Hospital-Aarhus University Hospital, Aalborg, Denmark

2. AdvanDx Inc., Woburn, Massachusetts

Abstract

ABSTRACT Rapid identification of four major pathogens from 1,231 positive blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes (AdvanDx Inc., Woburn, Mass.) was evaluated. For Escherichia coli , Staphylococcus aureus , and Candida albicans results agreed with conventional identification. The lower sensitivity of the Pseudomonas aeruginosa assay should not compromise the utility of the four assays.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.4.1947-1949.2005

Reference9 articles.

1. Gonzalez, V., E. Padilla, M. Gimenez, C. Vilaplana, A. Perez, G. Fernandez, M. D. Quesada, M. A. Pallares, and V. Ausina. 2004. Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH. Eur. J. Clin. Microbiol. Infect. Dis.23:396-398.

2. Kiska, D. L., and P. H. Gilligan. 2003. Pseudomonas, p. 719-728. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller and R. H. Yolken (ed.), Manual of clinical microbiology, 8th ed., vol. 1. ASM Press, Washington, D.C.

3. Leibovici, L., I. Shraga, M. Drucker, H. Konigsberger, Z. Samra, and S. D. Pitlik. 1998. The benefit of appropriate empirical antibiotic treatment in patients with bloodstream infection. J. Intern. Med.244:379-386.

4. Direct Identification of Staphylococcus aureus from Positive Blood Culture Bottles

5. Rapid Identification of Staphylococcus aureus Directly from Blood Cultures by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes

Cited by 49 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Colorimetric detection of Pseudomonas aeruginosa by aptamer-functionalized gold nanoparticles;Applied Microbiology and Biotechnology;2022-11-24

2. The role of Nucleic Acid Mimics (NAMs) on FISH-based techniques and applications for microbial detection;Microbiological Research;2022-09

3. Quality Control in Diagnostic Fluorescence In Situ Hybridization (FISH) in Microbiology;Methods in Molecular Biology;2021

4. 16S Genomics for Diagnosing Invasive Bacterial Infection;Reference Module in Biomedical Sciences;2021

5. Role of Rapid Diagnostics in Diagnosis and Management of Patients With Sepsis;The Journal of Infectious Diseases;2020-07-21