Misidentification of Mycobacterium leprae as Mycobacterium intracellulare by the COBAS AMPLICOR M. intracellulare Test-Reference-Cited by-同舟云学术

Misidentification of Mycobacterium leprae as Mycobacterium intracellulare by the COBAS AMPLICOR M. intracellulare Test

Published:2005-04 Issue:4 Volume:43 Page:1928-1929
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Lefmann M.¹²,Moter A.¹,Schweickert B.¹,Göbel U. B.¹

Affiliation:

1. Institut für Mikrobiologie und Hygiene, Campus Charité Mitte, Charité—Universitätsmedizin Berlin

2. Institut für Medizinische Mikrobiologie am HELIOS-Klinikum Berlin-Buch, HAEMA AG Berlin, Germany

Abstract

ABSTRACT Commercially available nucleic acid probe- and amplification-based systems for detection and differentiation of mycobacteria are widely used in clinical microbiology laboratories. Here we report two cases of human leprosy in which the COBAS AMPLICOR Mycobacterium intracellulare test led to false- positive results. Correct identification of Mycobacterium leprae was possible only by amplification and comparative sequence analysis of the 16S rRNA gene.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.4.1928-1929.2005

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