Direct Detection of Nocardia spp. in Clinical Samples by a Rapid Molecular Method

Author:

Couble Andrée1,Rodríguez-Nava Verónica1,de Montclos Michèle Pérouse2,Boiron Patrick1,Laurent Frédéric1

Affiliation:

1. UMR CNRS 5557, Center for Microbial Ecology, Opportunistic Pathogens and Environment Research Group, Observatoire Français des Nocardioses (OFN), Laboratoire de Mycologie Fondamentale et Appliquée aux Biotechnologies Industrielles, Faculté de Pharmacie, Université Claude Bernard Lyon I, Lyon

2. Laboratoire de Bactériologie, Centre Hospitalier Lyon Sud, Pierre Bénite, France

Abstract

ABSTRACT We developed a 16S PCR-based assay for the rapid detection of Nocardia spp. directly from human clinical samples. The applicability of the assay was confirmed by using 18 samples from patients with nocardiosis as diagnosed by conventional cultures and 20 clinical samples from patients with confirmed tuberculosis used as negative controls.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference27 articles.

1. Beaman, B. L. 1981. The possible role of L-phase variants of Nocardia in chronic infections. Zentbl. Bakteriol. Mikrobiol. Hyg. Abt. Suppl.11:221-227.

2. Beaman, B. L. 1982. Nocardiosis: role of the cell deficient state of Nocardia, p. 231-255. In G. J. Domingue (ed.), Cell wall defective bacteria: basic principles and clinical significance. Addison-Wesley Publishing Co., Inc., Reading, Mass.

3. Beaman, B. L. 1984. The cell wall as a determinant of pathogenicity in Nocardia: the role of L-forms in pathogenesis, p. 89-104. In L. Ortiz-Ortiz, L. F. Bojalil, and V. Yakoleff (ed.), Biological, biochemical and biomedical aspects of actinomycetes. Academic Press, Orlando, Fla.

4. Beaman, B. L., J. Burnside, B. Edwards, and W. Causey. 1976. Nocardial infections in the United States, 1972-1974. J. Infect. Dis.134:286-289.

5. Beaman, B. L., M. A. Saubolle, and R. J. Wallace. 1995. Nocardia, Rhodococcus, Streptomyces, Oerskovia, and other aerobic actinomycetes of medical importance, p. 379-399. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D.C.

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