Impact of Long-Term Storage on Stability of Standard DNA for Nucleic Acid-Based Methods-Reference-Cited by-同舟云学术

Impact of Long-Term Storage on Stability of Standard DNA for Nucleic Acid-Based Methods

Published:2010-11 Issue:11 Volume:48 Page:4260-4262
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Röder Barbara¹,Frühwirth Karin²,Vogl Claus³,Wagner Martin²,Rossmanith Peter¹

Affiliation:

1. Christian Doppler Laboratory for Molecular Food Analytics, Veterinärplatz 1, 1210 Vienna, Austria

2. Institute of Milk Hygiene, Milk Technology and Food Science, Department of Veterinary Public Health and Food Science, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria

3. Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria

Abstract

ABSTRACT Real-time PCR is dependent upon a calibration function for quantification. While long-term storage of standards saves cost and time, solutions of DNA are prone to degradation. We present here the benchmark treatment for preservation of DNA standards, involving storage in 50% glycerol-double-distilled water, whereby a deviation of 0.2 threshold cycle ( C T ) values resulted after 100 days of storage.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01230-10

Reference21 articles.

1. Anchordoquy, T. J., and M. C. Molina. 2007. Preservation of DNA. Cell Preserv. Technol. 5 : 180-188.

2. Bonner, G., and A. M. Klibanov. 2000. Structural stability of DNA in nonaqueous solvents. Biotechnol. Bioeng. 68 : 339-344.

3. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction

4. D'Agostino, M., M. Wagner, J. A. Vazquez-Boland, T. Kuchta, R. Karpiskova, J. Hoorfar, S. Novella, M. Scortti, J. Ellison, A. Murray, I. Fernandes, M. Kuhn, J. Pazlarova, A. Heuvelink, and N. Cook. 2004. A validated PCR-based method to detect Listeria monocytogenes using raw milk as a food model—towards an international standard. J. Food Prot. 67 : 1646-1655.

5. Davis, D. L., E. P. O'Brien, and C. M. Bentzley. 2000. Analysis of the degradation of oligonucleotide strands during the freezing/thawing processes using MALDI-MS. Anal. Chem. 72 : 5092-5096.

Cited by 70 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Rapid and visual detection of Mycoplasma genitalium using recombinase polymerase amplification combined with lateral flow strips;Journal of Microbiological Methods;2024-11

2. Development and validation of a 66K SNP array for the hard clam (Mercenaria mercenaria);BMC Genomics;2024-09-09

3. Monochrome Multiplex Quantitative PCR Telomere Length Measurement;Journal of Visualized Experiments;2024-03-22

4. High-throughput DNA synthesis for data storage;Chemical Society Reviews;2024

5. Rapid and Visual Detection of Mycoplasmoides Genitalium Using Recombinase Polymerase Amplification Combined with Lateral Flow Strips;2024