IS 6110 -Mediated Deletions of Wild-Type Chromosomes of Mycobacterium tuberculosis

Author:

Fang Z.1,Doig C.2,Kenna D. T.1,Smittipat N.3,Palittapongarnpim P.3,Watt B.2,Forbes K. J.1

Affiliation:

1. Medical Microbiology, Aberdeen University, Foresterhill, Aberdeen AB25 2ZD,1 and

2. Scottish Mycobacteria Reference Laboratory, The City Hospital, Edinburgh EH10 5SB,2 United Kingdom, and

3. Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand3

Abstract

ABSTRACT The ipl locus is a site for the preferential insertion of IS 6110 and has been identified as an insertion sequence, IS 1547 , in its own right. Various deletions around the ipl locus of clinical isolates of Mycobacterium tuberculosis were identified, and these deletions ranged in length from several hundred base pairs up to several kilobase pairs. The most obvious feature shared by these deletions was the presence of an IS 6110 copy at the deletion sites, which suggested two possible mechanisms for their occurrence, IS 6110 transposition and homologous recombination. To clarify the mechanism, an investigation was conducted; the results suggest that although deletion transpositionally mediated by IS 6110 was a possibility, homologous recombination was a more likely one. The implications of such chromosomal rearrangements for the evolution of M. tuberculosis , for IS 6110 -mediated mutagenesis, and for the development of genetic tools are discussed. The deletion of genomic DNA in isolates of M. tuberculosis has previously been noted at only a few sites. This study examined the deletional loss of genetic material at a new site and suggests that such losses may occur elsewhere too and may be more prevalent than was previously thought. Distinct from the study of laboratory-induced mutations, the detailed analysis of clinical isolates, in combination with knowledge of their evolutionary relationships to each other, gives us the opportunity to study mutational diversity in isolates that have survived in the human host and therefore offers a different perspective on the importance of particular genetic markers in pathogenesis.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3