Rapid, Radiolabeled-Microculture Method That Uses Macrophages for In Vitro Evaluation of Mycobacterium leprae Viability and Drug Susceptibility-Reference-Cited by-同舟云学术

Rapid, Radiolabeled-Microculture Method That Uses Macrophages for In Vitro Evaluation of Mycobacterium leprae Viability and Drug Susceptibility

Published:1983-04 Issue:4 Volume:17 Page:704-707
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Mittal A.¹,Sathish M.¹,Seshadri P. S.²,Nath Indira¹

Affiliation:

1. Department of Pathology, All-India Institute of Medical Sciences, New Delhi-110029, India

2. Central Leprosy Training Research Institute, Chingleput-603001, India

Abstract

This paper describes a microculture rapid assay using radiolabeling and mouse macrophages to determine the viability and the drug susceptibility or resistance of Mycobacterium leprae. Comparison of M. leprae resident macrophage cultures maintained in 96-well flat-bottomed plates showed results for viability and susceptibility or resistance to dapsone that were similar to results for concurrent cultures in Leighton tubes with greater numbers of bacilli and macrophages.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.17.4.704-707.1983

Reference10 articles.

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5. Uptake of 3H-thymidine in M. leprae inoculated mouse macrophage cultures as a rapid indicator of bacillary viability: factors influencing the specificity of the in vitro assay;Sathish M.;Int. J. Lepr.,1981

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