Identification and Characterization of Two Novel IS CR1 -Associated Genes, dfrA42 and dfrA43 , Encoding Trimethoprim-Resistant Dihydrofolate Reductases-Reference-Cited by-同舟云学术

Identification and Characterization of Two Novel IS CR1 -Associated Genes, dfrA42 and dfrA43 , Encoding Trimethoprim-Resistant Dihydrofolate Reductases

Published:2021-04-19 Issue:5 Volume:65 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Li Ling¹,Zhang Mengge¹,Wang Wenjia¹,Xia Ruirui¹,Ma Yanan¹,Wei Xin¹,Wang Xinhua²,Sun Xiaomin³,Xie Xiaohong⁴,Xie Shiling⁴,Wang Mingyu¹^ORCID,Xu Hai¹^ORCID

Affiliation:

1. State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, China

2. Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong, China

3. Environmental Research Institute, Shandong University, Qingdao, Shandong, China

4. Shandong Shtars Medical Technology Co., Ltd., Jinan, Shandong, China

Abstract

Two novel IS CR1 -associated dfr genes, dfrA42 and dfrA43 , were identified from trimethoprim (TMP)-resistant Proteus strains and were shown to confer high levels of TMP resistance (MIC, ≥1,024 mg/liter) when cloned into Escherichia coli isolates. These genes were hosted by complex class 1 integrons, suggesting their potential for dissemination.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Fundamental Research Fund of Shandong University

State Key Laboratory of Microbial Technology Open Project Funds, Shandong University

Key R&D Program of Shandong Province

Shandong Provincial Natural Science Foundation, China

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

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