Affiliation:
1. Food Laboratory, Microbiology Division, U.S. Army Natick Laboratories, Natick, Massachusetts 01760
Abstract
Spores of three strains each of type A and type B
Clostridium botulinum
were produced both by a biphasic (solid medium overlaid with an aqueous phase) and by a “conventional” (deep broth culture) procedure. Sporogenesis by the biphasic system was more rapid, convenient, and economical, and yielded as many or more heat-resistant (80 C, 10 min) spores per milliliter as by the conventional technique. Of several aqueous phases [thiamine-hydrochloride, yeast extract, (NH
4
)
2
SO
4
] tested with strain 62A, the highest spore colony counts were obtained with 2.0% (NH
4
)
2
SO
4
. The six strains formed maximum spore numbers in 5 to 6 days of incubation. Spores produced by the two methods had essentially equal radiation resistances (D and lag values), and their subcultures gave similar toxin titers (LD
50
values).
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference7 articles.
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5. Resistance of spores of Clostridium botulinum 33A to combinations of ultraviolet and gamma rays;Durban E.;App. Microbiol.,1969
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