Microculture Plaque Assay for Human and Simian Cytomegaloviruses

Author:

Chiba Shunzo1,Striker Robert L.1,Benyesh-Melnick Matilda1

Affiliation:

1. Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77025

Abstract

The plaque assay for human and simian cytomegaloviruses routinely carried out in 60-mm petri dishes (macrocultures) has been adapted for use in microcultures in flat-bottom 16-mm circular wells of disposable plastic trays. Virus titrations and serum neutralization assays carried out in microcultures yielded reproducible results that were identical to those obtained in macrocultures.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference11 articles.

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3. Spectrum of human cytomegalovirus complement-fixing antigens;Dreesman G. R.;J. Immunol.,1967

4. Complementrequiring neutralizing antibodies in hyperimmune sera to human cytomegaloviruses;Graham B. J.;J. Immunol.,1971

5. Preparation of specific antisera to cytomegaloviruses in goats;Haines H. G.;Proc. Soc. Exp. Biol. Med.,1971

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