Physical Map of the Channel Catfish Virus Genome: Location of Sites for Restriction Endonucleases Eco RI, Hin dIII, Hpa I, and Xba I

Abstract

The overall arrangement of nucleotide sequences in the DNA of channel catfish virus has been studied by cleavage with four restriction endonucleases. Physical maps have been developed for the location of sites for Eco RI, Hin dIII, Hpa I, and Xba I. The sum of the molecular weights of fragments generated by each restriction enzyme indicates a molecular weight of approximately 86 × 10 6 for the channel catfish virus genome. Fragments corresponding to the molecular ends of channel catfish virus DNA have been identified by their sensitivity to exonuclease treatment. The distribution of restriction sites in the genome shows that sequences included in a 12 × 10 6 -molecular weight region at one end are repeated with direct polarity at the other end, and that the overall genomic sequence order is nonpermuted.