Affiliation:
1. Department of Laboratory Medicine and Clinical Laboratory Center1 and
2. Laboratory of Drug Resistance in Bacteria,2 Gunma University School of Medicine, 3-39-22, Showa-machi, Maebashi 371-8511, Japan
Abstract
ABSTRACT
Acinetobacter baumannii
strains resistant to both imipenem (IPM) and ceftazidime (CAZ) were isolated from 1994 through 1996 at Gunma University Hospital. Nine isolates from different inpatients were examined for carbapenem-hydrolyzing activity and for the carbapemase gene
bla
IMP
by the PCR method. All nine isolates were carbapenemase-producing strains that hydrolyzed IPM and that harbored
bla
IMP
. The
bla
IMP
gene was transmissible by conjugation to an IPM-susceptible recipient strain of
A. baumannii
and conferred resistance to IPM, CAZ, cefotaxime (CTX), ampicillin (AMP), and piperacillin (PIP). Either intermediate or high-level resistance to amikacin (AMK) was transferred from two and five strains, respectively, concomitantly with
bla
IMP
, and gentamicin (GEN) resistance was also transferred in one instance of high-level AMK resistance. Comparative examination of clinical isolates for resistance patterns to nine drugs, IPM, CAZ, CTX, aztreonam, AMP, PIP, AMK, GEN, and norfloxacin, in addition to pulsed-field gel electrophoresis patterns with
Not
I-digested genomic DNA, confirmed nosocomial transmission of infections involving carbapenemase-producing
A. baumannii
strains.
Publisher
American Society for Microbiology
Cited by
66 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献