Affiliation:
1. Department of Preventive Dentistry, Kagoshima University Dental School, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan
Abstract
ABSTRACT
The genus
Abiotrophia
represents a heterogeneous group of fastidious cocci that show a dependence on pyridoxal hydrochloride analogs for growth. The genetic heterogeneity in the genus
Abiotrophia
was examined by DNA-DNA hybridization, PCR assay of genomic DNA sequences, and restriction fragment length polymorphism and sequence homology analyses of the PCR-amplified 16S rRNA gene. Nine type or reference strains of
Abiotrophia defectiva
,
Abiotrophia adiacens
, and
Abiotrophia elegans
and 36 oral
Abiotrophia
isolates including the ones presumptively identified as
Gemella morbillorum
by the rapid ID32 STREP system were divided into four groups:
A. defectiva
(genotype 1),
A. adiacens
(genotype 2),
A. elegans
(genotype 4), and a fourth species (genotype 3) which we propose be named
Abiotrophia para-adiacens
sp. nov. A PCR assay specific for detection and identification of the novel
Abiotrophia
species was developed.
A. para-adiacens
generally produced β-glucosidase but did not produce α- or β-galactosidase or arginine dihydrolase, did not ferment, trehalose, pullulan, or tagatose, and was serotype IV, V, or VI. Thus, it was distinguished phenotypically from
A. adiacens
,
A. elegans
, and
A. defectiva
as well as, apparently, from the recently described species
Abiotrophia balaenopterae
sp. nov., which produces arginine dihydrolase and which ferments pullulan but not sucrose (P. A. Lawson et al., Int. J. Syst. Bacteriol. 49:503–506, 1999). Strain ATCC 27527, currently listed as
G. morbillorum
, was a member of the species
A. para-adiacens.
Publisher
American Society for Microbiology
Cited by
38 articles.
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