Temperature-Sensitive Strain of Cryptococcus neoformans Producing Hyphal Elements in a Feline Nasal Granuloma

Author:

Bemis D. A.1,Krahwinkel D. J.2,Bowman L. A.3,Mondon P.4,Kwon-Chung K. J.4

Affiliation:

1. Department of Comparative Medicine,1

2. Department of Small Animal Clinical Sciences,2 and

3. Department of Pathology,3 College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee 37996, and

4. Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 208924

Abstract

ABSTRACT We report the isolation of a temperature-sensitive, serotype A, mating type α strain of Cryptococcus neoformans from a case of nasal cryptococcosis in a cat. The strain grew extremely slowly at 35°C and failed to grow at 37°C in vitro. Histopathological sections of the infected tissue revealed yeast cells producing hyphae up to several hundred micrometers in length, in addition to numerous encapsulated yeast cells typical of C. neoformans . The cultures grown on yeast extract-peptone-glucose agar at 35°C also produced some yeast cells with germ tube-like hyphal elements up to 100 μm in length.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference9 articles.

1. Jacobs G. J. Medleau L. Cryptococcus Infectious diseases of the dog and cat. W. B. Greene C. E. 1998 383 390 Saunders Company Philadelphia Pa

2. Cryptococcal infection in cats: factors influencing treatment outcome, and results of sequential serum antigen titers in 35 cats;Jacobs G. J.;J. Vet. Intern. Med.,1997

3. Kwon-Chung K. J. Bennett J. E. Medical mycology 1992 396 446 Lea & Febiger Philadelphia Pa

4. Cryptococcosis in cats: clinical and mycological assessment of 29 cases and evaluation of treatment using orally administered fluconazole;Malik R.;J. Med. Vet. Mycol.,1992

5. Aids-related cryptococcosis: diagnostic aspects, prognostic and therapeutic implications;Manfredi R.;Ann. Ital. Med. Int.,1998

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