A Method To Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays-Reference-Cited by-同舟云学术

A Method To Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays

Published:2020-05-26 Issue:6 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Wang Qiang¹²³,Du Qin¹³,Guo Bin¹²³,Mu Daiyong⁴,Lu Xiaolan¹³,Ma Qiang¹²³,Guo Yangliu¹²³,Fang Li¹²³,Zhang Bing⁴,Zhang Guoyuan¹³,Guo Xiaolan¹²³

Affiliation:

1. Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China

2. Faculty of Laboratory Medicine, North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China

3. Center for Translational Medicine, North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China

4. Department of Laboratory Medicine, Nanchong Central Hospital, Nanchong, Sichuan, People’s Republic of China

Abstract

We set out to investigate the interference factors that led to false-positive novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgM detection results using gold immunochromatography assay (GICA) and enzyme-linked immunosorbent assay (ELISA) and the corresponding solutions. GICA and ELISA were used to detect SARS-CoV-2 IgM in 86 serum samples, including 5 influenza A virus (Flu A) IgM-positive sera, 5 influenza B virus (Flu B) IgM-positive sera, 5 Mycoplasma pneumoniae IgM-positive sera, 5 Legionella pneumophila IgM-positive sera, 6 sera of HIV infection patients, 36 rheumatoid factor IgM (RF-IgM)-positive sera, 5 sera from hypertensive patients, 5 sera from diabetes mellitus patients, and 14 sera from novel coronavirus infection disease 19 (COVID-19) patients.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00375-20

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