Complex of Virus-Like Particles Containing Double-Stranded RNA from Thielaviopsis basicola

Author:

Bozarth R. F.1,Goenaga A.1

Affiliation:

1. Boyce Thompson Institute, Yonkers, New York 10701

Abstract

Six randomly selected isolates of Thielaviopsis basicola were found to contain spherical virus-like particles (VLPs) approximately 40 nm in diameter. One isolate, ATCC 34114, selected for further analysis contained a complex of VLPs that sedimented as eight or more bands in sucrose density gradients and contained five size classes of double-stranded RNA. Five discrete precipitation lines were obtained in immunoelectrophoresis, which indicated that this isolate of T. basicola contains five distinct species of VLPs.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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