The Carboxy-Terminal Segment of the Human Cytomegalovirus DNA Polymerase Accessory Subunit UL44 Is Crucial for Viral Replication-Reference-Cited by-同舟云学术

The Carboxy-Terminal Segment of the Human Cytomegalovirus DNA Polymerase Accessory Subunit UL44 Is Crucial for Viral Replication

Published:2010-11 Issue:21 Volume:84 Page:11563-11568
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Silva Laurie A.¹,Loregian Arianna²,Pari Gregory S.³,Strang Blair L.¹,Coen Donald M.¹

Affiliation:

1. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115

2. Department of Histology, Microbiology, and Medical Biotechnologies, University of Padua, 35121 Padua, Italy

3. Department of Microbiology and Immunology and Cell and Molecular Biology Graduate Program, University of Nevada—Reno, Reno, Nevada 89557

Abstract

ABSTRACT The amino-terminal 290 residues of UL44, the presumed processivity factor of human cytomegalovirus DNA polymerase, possess all of the established biochemical activities of the full-length protein, while the carboxy-terminal 143 residues contain a nuclear localization signal (NLS). We found that although the amino-terminal domain was sufficient for origin-dependent synthesis in a transient-transfection assay, the carboxy-terminal segment was crucial for virus replication and for the formation of DNA replication compartments in infected cells, even when this segment was replaced with a simian virus 40 NLS that ensured nuclear localization. Our results suggest a role for this segment in viral DNA synthesis.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01033-10

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