Production of Minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800-Reference-Cited by-同舟云学术

Production of Minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800

Published:2004-09 Issue:9 Volume:70 Page:5704-5707
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Cho Hee-Yeon¹,Yukawa Hideaki²,Inui Masayuki²,Doi Roy H.¹,Wong Sui-Lam³

Affiliation:

1. Section of Molecular and Cellular Biology, University of California, Davis, California

2. Research Institute of Innovative Technology for the Earth, Kyoto, Japan

3. Department of Biological Sciences, The University of Calgary, Calgary, Alberta, Canada

Abstract

ABSTRACT Two genes encoding EngB endoglucanase and mini-CbpA1 scaffolding protein of Clostridium cellulovorans were constructed and coexpressed in Bacillus subtilis WB800. The resulting minicellulosomes were isolated by gel filtration chromatography and characterized. Biochemical and immunological evidence indicated that fraction II contained minicellulosomes consisting of mini-CbpA1 and EngB. The in vivo synthesis of minicellulosomes suggests that it will be possible in the future to insert into B. subtilis cellulosomal genes that will allow growth on cellulosic materials and the production of various designer cellulosomes with specific functions.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.9.5704-5707.2004

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