Affiliation:
1. Laboratory of Microbiology
2. CMBI, Nijmegen, The Netherlands
3. Agrotechnology and Food Innovations, Wageningen University and Research Centre, Wageningen
Abstract
ABSTRACT
Clostridium acetobutylicum
ATCC 824 is a solventogenic bacterium that grows heterotrophically on a variety of carbohydrates, including glucose, cellobiose, xylose, and lichenan, a linear polymer of β-1,3- and β-1,4-linked β-
d
-glucose units.
C. acetobutylicum
does not degrade cellulose, although its genome sequence contains several cellulase-encoding genes and a complete cellulosome cluster of cellulosome genes. In the present study, we demonstrate that a low but significant level of induction of cellulase activity occurs during growth on xylose or lichenan. The
celF
gene, located in the cellulosome-like gene cluster and coding for a unique cellulase that belongs to glycoside hydrolase family 48, was cloned in
Escherichia coli
, and antibodies were raised against the overproduced CelF protein. A Western blot analysis suggested a possible catabolite repression by glucose or cellobiose and an up-regulation by lichenan or xylose of the extracellular production of CelF by
C. acetobutylicum
. Possible reasons for the apparent inability of
C. acetobutylicum
to degrade cellulose are discussed.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
45 articles.
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