Characterization of Two Paenibacillus amylolyticus Strain 27C64 Pectate Lyases with Activity on Highly Methylated Pectin-Reference-Cited by-同舟云学术

Characterization of Two Paenibacillus amylolyticus Strain 27C64 Pectate Lyases with Activity on Highly Methylated Pectin

Published:2010-09 Issue:17 Volume:76 Page:6006-6009
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Boland Whitney E.¹,Henriksen Emily DeCrescenzo¹,Doran-Peterson Joy¹

Affiliation:

1. Department of Microbiology, University of Georgia, Athens, Georgia 30602

Abstract

ABSTRACT Two pectate lyases were identified from Paenibacillus amylolyticus 27C64; both enzymes demonstrated activity on methylated pectin in addition to polygalacturonic acid. PelA is in a subclass of the pectate lyase family III. PelB shows some features of pectate lyase family I but is highly divergent.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00043-10

Reference25 articles.

1. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

2. Berensmeier, S., S. A. Singh, J. Meens, and K. Buchholz. 2004. Cloning of the pelA gene from Bacillus licheniformis 14A and biochemical characterization of recombinant, thermostable, high-alkaline pectate lyase. Appl. Biochem. Biotechnol.64:560-567.

3. Collmer, A., J. L. Ried, and M. S. Mount. 1988. Assay methods for pectic enzymes. Methods Enzymol.161:329-335.

4. Microbial enzymes and biotechnology II. 1990

5. Doran, J. B., J. Cripe, M. Sutton, and B. Foster. 2000. Fermentations of pectin-rich biomass with recombinant bacteria to produce fuel ethanol. Appl. Biochem. Biotechnol.86:141-152.

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