Affiliation:
1. Life Sciences Laboratory, Northrop Corporate Laboratories, Hawthorne, California 90250
Abstract
The europium chelate of 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione (thenoyl-trifluoroacetone; TTA) is firmly bound to microorganisms. It fluoresces brightly at 613 nm with activation at 340 nm. Cells may be stained with 10
−3
m
chelate in 50% ethyl alcohol, followed by washing with 50% ethyl alcohol. Equal or better stains are produced with 10
−3
m
aqueous europium salt, water wash, and 10
−2
m
aqueous TTA. A noncomplexing buffer should be used to maintain the
p
H at 6.5 to 6.8.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference4 articles.
1. Ohnesorge W. E. 1966. Fluorescence of metal chelate compounds p. 151-167. In D. M. Hercules (ed.) Fluorescence and phosphorescence analysis. Interscience New York.
2. Stem K. G. 1956. Interaction of rare-earth compounds with substances of biological interest p. 143-162. In G. C. Kyker and E. B. Anderson (ed.) Rare earths in biochemical and medical research. Oak Ridge Institute for Nuclear Studies Oak Ridge Tenn.
3. Desoxyribonucleic acid complexes of rare earths;Stem K. G.;Biochim. Biophys. Acta,1953
4. Intramolecular energy transfer. The fluorescence of complexes of europium;Weissman S. I.;J. Chem. Phys.,1942
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