Author:
Schuchmann Kai,Schmidt Silke,Martinez Lopez Antonio,Kaberline Christina,Kuhns Martin,Lorenzen Wolfram,Bode Helge B.,Joos Friederike,Müller Volker
Abstract
Acetogenic bacteria can grow by the oxidation of various substrates coupled to the reduction of CO2in the Wood-Ljungdahl pathway. Here, we show that growth of the acetogenAcetobacterium woodiion 1,2-propanediol (1,2-PD) as the sole carbon and energy source is independent of acetogenesis. Enzymatic measurements and metabolite analysis revealed that 1,2-PD is dehydrated to propionaldehyde, which is further oxidized to propionyl coenzyme A (propionyl-CoA) with concomitant reduction of NAD. NADH is reoxidized by reducing propionaldehyde to propanol. The potential gene cluster coding for the responsible enzymes includes genes coding for shell proteins of bacterial microcompartments. Electron microscopy revealed the presence of microcompartments as well as storage granules in cells grown on 1,2-PD. Gene clusters coding for the 1,2-PD pathway can be found in other acetogens as well, but the distribution shows no relation to the phylogeny of the organisms.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
28 articles.
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