Demonstration of Polysaccharide Capsule in Campylobacter jejuni Using Electron Microscopy

Author:

Karlyshev Andrey V.1,McCrossan Maria V.1,Wren Brendan W.1

Affiliation:

1. Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, London WC1E 7HT, United Kingdom

Abstract

ABSTRACT Recently, we reported that Campylobacter jejuni , an important gastrointestinal pathogen, has the genetic determinants to produce a capsular polysaccharide (Karlyshev et al., Mol. Microbiol. 35:529–541, 2000). Despite these data, the presence of a capsule in these bacteria has remained controversial. In this study we stain C. jejuni cells with the cationic dye Alcian blue and demonstrate for the first time by electron microscopy that C. jejuni cells produce a polysaccharide capsule that is retained in the coccoid form but is absent in a kpsM mutant.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference20 articles.

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5. Electron microscopic visualization of the capsule of Pasteurella multocida;Gunther H.;Acta Histochem. Suppl.,1986

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