Molecular Dissection of Interactions between Rad51 and Members of the Recombination-Repair Group

Author:

Krejci Lumir12,Damborsky Jiri3,Thomsen Bo2,Duno Morten2,Bendixen Christian2

Affiliation:

1. Department of Analysis of Biologically Important Molecular Complexes, Masaryk University, 612 65 Brno, 1 and

2. Department of Breeding and Genetics, Section of Molecular Genetics, Research Center Foulum, DK-8830 Tjele, Denmark2

3. Laboratory of Biomolecular Structure and Dynamics, Masaryk University, 611 37 Brno, 3 Czech Republic, and

Abstract

ABSTRACT Recombination is important for the repair of DNA damage and for chromosome segregation during meiosis; it has also been shown to participate in the regulation of cell proliferation. In the yeast Saccharomyces cerevisiae , recombination requires products of the RAD52 epistasis group. The Rad51 protein associates with the Rad51, Rad52, Rad54, and Rad55 proteins to form a dynamic complex. We describe a new strategy to screen for mutations which cause specific disruption of the interaction between certain proteins in the complex, leaving other interactions intact. This approach defines distinct protein interaction domains and protein relationships within the Rad51 complex. Alignment of the mutations onto the constructed three-dimensional model of the Rad51 protein reveal possible partially overlapping interfaces for the Rad51-Rad52 and the Rad51-Rad54 interactions. Rad51-Rad55 and Rad51-Rad51 interactions are affected by the same spectrum of mutations, indicating similarity between the two modes of binding. Finally, the detection of a subset of mutations within Rad51 which disrupt the interaction with mutant Rad52 protein but activate the interaction with Rad54 suggests that dynamic changes within the Rad51 protein may contribute to an ordered reaction process.

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Reference57 articles.

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3. A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae;Bai Y.;Genes Dev.,1996

4. A yeast mating-selection scheme for detection of protein-protein interactions;Bendixen C.;Nucleic Acids Res.,1994

5. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance;Boeke J. D.;Mol. Gen. Genet.,1984

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