Affiliation:
1. INSERM U-411, CHU Necker-Enfants Malades, 75730 Paris Cedex 15, France
Abstract
ABSTRACT
Listeria monocytogenes
is a gram-positive, facultative intracellular pathogen that can cause severe food-born infections in humans and animals. We have adapted signature-tagged transposon mutagenesis to
L. monocytogenes
to identify new genes involved in virulence in the murine model of infection. We used transposon Tn
1545
carried on the integrative vector pAT113. Forty-eight tagged transposons were constructed and used to generate banks of
L. monocytogenes
mutants. Pools of 48 mutants were assembled, taking one mutant from each bank, injected into mice, and screened for those affected in their multiplication in the brains of infected animals. From 2,000 mutants tested, 18 were attenuated in vivo. The insertions harbored by these mutants led to the identification of 10 distinct loci, 7 of which corresponded to previously unknown genes. The properties of four loci involving putative cell wall components were further studied in vitro and in vivo. The data suggested that these components are involved in bacterial invasion and multiplication in the brain.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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