Affiliation:
1. Departments of Medicine
2. Microbiology and Molecular Genetics, University of Vermont College of Medicine, Burlington, Vermont 05405
Abstract
ABSTRACT
Entamoeba histolytica
, the cause of amebiasis, is believed to have no continuous endoplasmic reticulum (ER), with ER functions occurring in vesicles. Here, using an ER-targeted green fluorescent protein fusion protein and fluorescence loss in photobleaching, we have unambiguously demonstrated the presence of a continuous ER compartment in living
E. histolytica
trophozoites.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Reference21 articles.
1. Anonymous. 1997. WHO/PAHO/UNESCO report. A consultation with experts on amoebiasis. Mexico City, Mexico 28-29 January, 1997. Epidemiol. Bull.18:13-14.
2. Bakker-Grunwald, T., and C. Wostmann. 1993. Entamoeba histolytica as a model for the primitive eukaryotic cell. Parasitol. Today9:27-31.
3. Clark, C. G., and A. J. Roger. 1995. Direct evidence for secondary loss of mitochondria in Entamoeba histolytica. Proc. Natl. Acad. Sci. USA92:6518-6521.
4. Cole, N. B., C. L. Smith, N. Sciaky, M. Terasaki, M. Edidin, and J. Lippincott-Schwartz. 1996. Diffusional mobility of Golgi proteins in membranes from living cells. Science273:797-801.
5. Dayel, M. J., and A. S. Verkman. 1999. Diffusion of green fluorescent protein in the aqueous-phase lumen of the endoplasmic reticulum. Biophys. J.76:2843-2851.
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