Affiliation:
1. Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0308
Abstract
ABSTRACT
The
Methanococcus jannaschii
gene MJ1392 was cloned, and its protein product was hyperexpressed in
Escherichia coli
. The resulting protein was purified and shown to catalyze the condensation of pyruvate and acetyl coenzyme A, with the formation of (
R
)-citramalate. Thus, this gene (
cimA
) encodes an (
R
)-citramalate synthase (CimA). This is the first identification of this enzyme, which is likely involved in the biosynthesis of isoleucine.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
86 articles.
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