Affiliation:
1. Lehrstuhl für Mikrobiologie, Biozentrum der Universität Würzburg, 97074 Würzburg,1 and
2. Department of Microbiology, University of Washington, Seattle, Washington 981952
3. CREATOGEN GmbH, 86156 Augsburg,3 Germany, and
Abstract
ABSTRACT
Salmonella typhimurium
causes systemic and fatal infection in inbred mice, while the related serotype
Salmonella typhi
is avirulent for mammals other than humans. In order to identify genes from the virulent strain
S. typhimurium
ATCC 14028 that are absent in
S. typhi
Ty2, and therefore might be involved in
S. typhimurium
mouse virulence, a PCR-supported genomic subtractive hybridization procedure was employed. We have identified a novel putative fimbrial operon,
stfACDEFG
, located at centisome 5 of the
S. typhimurium
chromosome, which is absent in
S. typhi
,
Salmonella arizonae
, and
Salmonella bongori
but was detected in several other
Salmonella
serotypes. The fimbrial genes represent a genomic insertion in
S. typhimurium
compared to the respective region between
fhuB
and
hemL
in
Escherichia coli
K-12. In addition, the subtraction procedure yielded F plasmid-related sequences from the
S. typhimurium
virulence plasmid, a number of DNA fragments representing parts of lambdoid prophages and putative sugar transporters, and several fragments with unknown sequences. The majority of subtracted chromosomal sequences map to three distinct locations, around centisomes 5, 27, and 57.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
43 articles.
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