Characterization of MarR Superrepressor Mutants-Reference-Cited by-同舟云学术

Characterization of MarR Superrepressor Mutants

Published:1999-05-15 Issue:10 Volume:181 Page:3303-3306
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Alekshun Michael N.¹²,Levy Stuart B.¹²³

Affiliation:

1. Center for Adaptation Genetics and Drug Resistance,1 and

2. Departments of Molecular Biology and Microbiology2 and of

3. Medicine,3 Tufts University School of Medicine, Boston, Massachusetts 02111

Abstract

ABSTRACT MarR negatively regulates expression of the multiple antibiotic resistance ( mar ) locus in Escherichia coli . Superrepressor mutants, generated in order to study regions of MarR required for function, exhibited altered inducer recognition properties in whole cells and increased DNA binding to marO in vitro. Mutations occurred in three areas of the relatively small MarR protein (144 amino acids). It is surmised that superrepression results from increased DNA binding activities of these mutant proteins.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.181.10.3303-3306.1999

Reference22 articles.

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3. Alekshun M. N. and S. B. Levy. Unpublished data.

4. Repressor mutations in the marRAB operon that activate oxidative stress genes and multiple antibiotic resistance in Escherichia coli

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