Regulation of Alginate Biosynthesis in Pseudomonas syringae pv. syringae

Abstract

ABSTRACT Both Pseudomonas aeruginosa and the phytopathogen P. syringae produce the exopolysaccharide alginate. However, the environmental signals that trigger alginate gene expression in P. syringae are different from those in P. aeruginosa with copper being a major signal in P. syringae . In P. aeruginosa , the alternate sigma factor encoded by algT (ς 22 ) and the response regulator AlgR1 are required for transcription of algD , a gene which encodes a key enzyme in the alginate biosynthetic pathway. In the present study, we cloned and characterized the gene encoding AlgR1 from P. syringae . The deduced amino acid sequence of AlgR1 from P. syringae showed 86% identity to its P. aeruginosa counterpart. Sequence analysis of the region flanking algR1 in P. syringae revealed the presence of argH , algZ , and hemC in an arrangement virtually identical to that reported in P. aeruginosa . An algR1 mutant, P. syringae FF5.32, was defective in alginate production but could be complemented when algR1 was expressed in trans . The algD promoter region in P. syringae ( PsalgD ) was also characterized and shown to diverge significantly from the algD promoter in P. aeruginosa . Unlike P. aeruginosa , algR1 was not required for the transcription of algD in P. syringae , and PsalgD lacked the consensus sequence recognized by AlgR1. However, both the algD and algR1 upstream regions in P. syringae contained the consensus sequence recognized by ς 22 , suggesting that algT is required for transcription of both genes.