Affiliation:
1. Laboratory of Microbiology, Department of Biomolecular Sciences, Wageningen Agricultural University, NL-6703 CT Wageningen, The Netherlands,1 and
2. Institut für Allgemeine Mikrobiologie der Christian-Albrechts-Universität zu Kiel, D-24118 Kiel, Germany2
Abstract
ABSTRACT
The genetic organization, expression, and regulation of the
celB
locus of the hyperthermophilic archaeon
Pyrococcus furiosus
were analyzed. This locus includes the
celB
gene, which codes for an intracellular β-glucosidase, and a divergently orientated gene cluster,
adhA-adhB-lamA
, which codes for two alcohol dehydrogenases and an extracellular β-1,3-endoglucanase that is transcribed as a polycistronic messenger (the
lamA
operon). During growth of
P. furiosus
on either the β-1,4-linked glucose dimer cellobiose or the β-1,3-linked glucose polymer laminarin, the activities of both β-glucosidase and endoglucanase were increased at least fivefold compared with levels during growth on maltose or pyruvate. Northern blot analysis revealed an enhanced transcription of both the
celB
gene and the
lamA
operon in the presence of these glucose-containing substrates. The in vivo and in vitro transcription initiation sites of both the
celB
gene and the
lamA
operon were identified 25 nucleotides downstream of conserved TATA box motifs. A number of repeating sequences have been recognized in the
celB-adhA
intergenic region, some of which might be part of a transcriptional regulator-binding site.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
23 articles.
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