Affiliation:
1. Groupe “Réparation des Lésions Radio-et Chimio-Induites,” UMR 1772 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France
Abstract
ABSTRACT
Deinococcus radiodurans
is able to resist and survive extreme DNA damage induced by ionizing radiation and many other DNA-damaging agents. It is believed that it possesses highly efficient DNA repair mechanisms. To characterize the repair pathway of oxidized purines in this bacteria, we have purified, from crude extracts, proteins that recognize these oxidized bases. We report here that
D. radiodurans
possesses two proteins excising the oxidized purines (formamidopyrimidine and 8-oxoguanine) by a DNA glycosylase–a purinic/apyrimidine lyase mechanism. Moreover, one of those proteins is endowed with a thymine glycol DNA glycosylase activity. One of these proteins could be the homolog of the
Escherichia coli
Fpg enzyme, which confirms the existence of a base excision repair system in this bacteria.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference45 articles.
1. Studies on a radio-resistant micrococcus. I. Isolation, morphology, cultural characteristics, and resistance to gamma radiation;Anderson A. W.;Food Technol.,1956
2. Purification and characterization of Escherichia coli endonuclease III from the cloned nth gene;Asahara H.;Biochemistry,1989
3. Two rotameric forms of imidazole ring-opened 7-methylguanine are present in alkylated polynucleotides;Boiteux S.;Nucleic Acids Res.,1984
4. Two rotameric forms of imidazole ring-opened 7-methylguanine are present in alkylated polynucleotides;Boiteux S.;Nucleic Acids Res.,1984
5. Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein;Boiteux S.;EMBO J.,1987
Cited by
27 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献