Membrane-Bound Pyrroloquinoline Quinone-Dependent Dehydrogenase in Gluconobacter oxydans M5, Responsible for Production of 6-(2-Hydroxyethyl) Amino-6-Deoxy- l -Sorbose-Reference-Cited by-同舟云学术

Membrane-Bound Pyrroloquinoline Quinone-Dependent Dehydrogenase in Gluconobacter oxydans M5, Responsible for Production of 6-(2-Hydroxyethyl) Amino-6-Deoxy- l -Sorbose

Published:2008-08-15 Issue:16 Volume:74 Page:5250-5253
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Yang Xue-Peng¹²,Wei Liu-Jing¹,Lin Jin-Ping¹,Yin Bo¹,Wei Dong-Zhi¹

Affiliation:

1. State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China

2. College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China

Abstract

ABSTRACT A membrane-bound protein purified from Gluconobacter oxydans M5 was confirmed to be a pyrroloquinoline quinone-dependent d -sorbitol dehydrogenase. Gene disruption and complementation experiments demonstrated that this enzyme is responsible for the oxidation of 1-(2-hydroxyethyl) amino- 1 -deoxy- d -sorbitol (1NSL) to 6-(2-hydroxyethyl) amino-6-deoxy- l -sorbose (6NSE), which is the precursor of an antidiabetic drug, miglitol.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00272-08

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