Affiliation:
1. Department of Microbiology, The Technical University of Denmark, DK-2800 Lyngby, Denmark
Abstract
ABSTRACT
The binding of DnaA protein to its DNA binding sites—DnaA boxes—in the chromosomal
oriC
region is essential for initiation of chromosome replication. In this report, we show that additional DnaA boxes affect chromosome initiation control, i.e., increase the initiation mass. The cellular DnaA box concentration was increased by introducing pBR322-derived plasmids carrying DnaA boxes from the
oriC
region into
Escherichia coli
and by growing the strains at different generation times to obtain different plasmid copy numbers. In fast-growing cells, where the DnaA box plasmid copy number per
oriC
locus was low, the presence of extra DnaA boxes caused only a moderate increase in the initiation mass. In slowly growing cells, where the DnaA box plasmid copy number per
oriC
locus was higher, we observed more pronounced increases in the initiation mass. Our data clearly show that the presence of extra DnaA boxes increases the initiation mass, supporting the idea that the initiation mass is determined by the normal complement of DnaA protein binding sites in
E. coli
cells.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
44 articles.
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