Multiple Single-Nucleotide Polymorphism Detection for Antimalarial Pyrimethamine Resistance via Allele-Specific PCR Coupled with Gold Nanoparticle-Based Lateral Flow Biosensor

Author:

Jiang Tingting12,Huang Yan3,Cheng Weijia12,Sun Yifei12,Wei Wei3,Wu Kai4,Shen Chen12,Fu Xiaolong12,Dong Haifeng3,Li Jian12ORCID

Affiliation:

1. Department of Human Parasitology, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, China

2. Department of Infectious Diseases, Renmin Hospital, Hubei University of Medicine, Shiyan, China

3. Beijing Key Laboratory for Bioengineering and Sensing Technology, Research Centre for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science & Technology, Beijing, China

4. Department of Schistosomiasis and Endemic Diseases, Wuhan City Center for Disease Prevention and Control, Wuhan, China

Abstract

Molecular genotyping holds tremendous potential to detect antimalarial drug resistance (ADR) related to single nucleotide polymorphisms (SNPs). However, it relies on the use of complicated procedures and expensive instruments.

Funder

Foundation for Innovation Research Team of Hubei University of Medicine

Innovative Research Program for Graduates of Hubei University of Medicine

Basic Research Business Fund of Central Universities

National Natural Science Foundation of China

China Postdoctoral Science Foundation

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference35 articles.

1. WHO. World malaria report. 2019. World Health Organization, Geneva, Switzerland.

2. Persistence of Sulfadoxine-Pyrimethamine Resistance Despite Reduction of Drug Pressure in Malawi

3. WHO Global Malaria Programme, WHO Department of Reproductive Health and Research, WHO Department of Maternal, Newborn, Child and Adolescent Health. 2013. WHO policy brief for the implementation of intermittent preventive treatment of malaria in pregnancy using sulfadoxine-pyrimethamine (IPTp-SP). World Health Organization, Geneva, Switzerland.

4. Point mutations in the dihydrofolate reductase-thymidylate synthase gene as the molecular basis for pyrimethamine resistance in Plasmodium falciparum

5. Allelic exchange at the endogenous genomic locus in Plasmodium falciparum proves the role of dihydropteroate synthase in sulfadoxine-resistant malaria

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