Affiliation:
1. Department of Biological Sciences, State University of New York, Binghamton, New York 13902
Abstract
ABSTRACT
rRNA gene sequencing and PCR assays indicated that 215 isolates of root nodule bacteria from two
Mimosa
species at three sites in Costa Rica belonged to the genera
Burkholderia
,
Cupriavidus
, and
Rhizobium
. This is the first report of
Cupriavidus
sp. nodule symbionts for
Mimosa
populations within their native geographic range in the neotropics.
Burkholderia
spp. predominated among samples from
Mimosa pigra
(86% of isolates), while there was a more even distribution of
Cupriavidus
,
Burkholderia
, and
Rhizobium
spp. on
Mimosa pudica
(38, 37, and 25% of isolates, respectively). All
Cupriavidus
and
Burkholderia
genotypes tested formed root nodules and fixed nitrogen on both
M. pigra
and
M. pudica
, and sequencing of rRNA genes in strains reisolated from nodules verified identity with inoculant strains. Inoculation tests further indicated that both
Cupriavidus
and
Burkholderia
spp. resulted in significantly higher plant growth and nodule nitrogenase activity (as measured by acetylene reduction assays) relative to plant performance with strains of
Rhizobium
. Given the prevalence of
Burkholderia
and
Cupriavidus
spp. on these
Mimosa
legumes and the widespread distribution of these plants both within and outside the neotropics, it is likely that both β-proteobacterial genera are more ubiquitous as root nodule symbionts than previously believed.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
126 articles.
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