mRNA-Based Parallel Detection of Active Methanotroph Populations by Use of a Diagnostic Microarray-Reference-Cited by-同舟云学术

mRNA-Based Parallel Detection of Active Methanotroph Populations by Use of a Diagnostic Microarray

Published:2006-02 Issue:2 Volume:72 Page:1672-1676
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bodrossy Levente¹,Stralis-Pavese Nancy¹,Konrad-Köszler Marianne¹,Weilharter Alexandra¹,Reichenauer Thomas G.²,Schöfer David²,Sessitsch Angela¹

Affiliation:

1. Department of Bioresources

2. Department of Environmental Research, ARC Seibersdorf Research GmbH, A-2444 Seibersdorf, Austria

Abstract

ABSTRACT A method was developed for the mRNA-based application of microbial diagnostic microarrays to detect active microbial populations. DNA- and mRNA-based analyses of environmental samples were compared and confirmed via quantitative PCR. Results indicated that mRNA-based microarray analyses may provide additional information on the composition and functioning of microbial communities.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.72.2.1672-1676.2006

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